HarvestPlus handbook for carotenoid analysis

Carotenoid analysis is inherently difficult and error prone. Despite substantial
improvements and refinements in the methods for analyzing these fascinating
but complicated compounds, discrepancies in analytical data can still be
perceived in the literature. Analysts must be well informed about the nature and
properties of carotenoids and the problems associated with their identification
and quantification.

Carotenoid analysis consists of several steps that can be carried out efficiently
in various ways. Thus, in this handbook several procedures are given for some
steps to enable the analyst to choose the procedure that best suits his/her laboratory
conditions and experience. These procedures have been put together and
evaluated for HarvestPlus crops in our laboratory, but we recognize that other
procedures may also produce reliable results. It is important that analysts test
the methods in their own laboratory prior to performing the actual analyses.
Knowledge of the purpose and possible sources of error in each step will assist
the analyst in appraising the performances of the methods. Indeed, the key
element in the accurate determination of carotenoids is undoubtedly the analyst.
Even a well-validated method may perform badly in the hands of an analyst who
does not seek the appropriate information and does not pay attention to the
many important details that are necessary to ensure the accuracy of the
analytical results.

Download errata file

Author: 
Rodriguez-Amaya, Delia B.
Kimura, Mieko
Published date: 
2004
Publisher: 
International Center for Tropical Agriculture (CIAT)
Series number: 
2
PDF file: